DIFFERENTIAL PULSE VOLTAMMETRIC DETERMINATION OF GUANOSINE AND 8-HYDROXYGUANOSINE

Abstract

Purine nucleosides are well known for their metabolic and biological effects on human beings. The development of sophisticated techniques in electrochemistry and growing importance of oxidation reactions in biological systems facilitated determination of two or more compounds simultaneously. The electrochemical studies of biomolecules provide useful and valuable information about their biological redox reactions. Electrochemical oxidation of guanosine and 8-hydroxyguanosine has been carried out at Pyrolytic Graphite Electrode by differential pulse voltammetry in the pH range 2.5-10.3. IF, versus concentration studies at pH 7 suggested that guanosine and 8-hydroxyguanosine can be determined simultaneously using differential pulse voltammetry in the concentration range of 0.1 to 0.0005 mM.