EFFECT OF CARBOHYDRATES, AMINO ACIDS, AND PEPTONES ON SF-9 CELL CULTURE IN TUBESPIN BIOREACTORS

Abstract

Lepidopteran insect cell culture technology has progressed to the point of becoming an essential part of one of the most successful eukaryotic expression systems and is increasingly used industrially on a large scale. Therefore, there is a constant need for convenient and low-cost culture media capable of supporting good insect cell growth and ensuring high yield of baculovirus as well as the strong expression of recombinant proteins. This study is performed to alter growth characteristics of Sf-9 cells with simple supplementation of basal IPL-41 with carbohydrates, amino acids and peptones. These compounds are an essential part of the insect cell metabolism. The process of this simple media development is highly cost effective and aids to obtain cell cultures with higher cell densities, superior viabilities and longer culture viabilities