Insight into the lipid augmentation characteristics of oleaginous yeast grown under sea water and wastewater medium

Abstract

Increasing demand of fossil fuels impels scientist to search for new energy sources. Plants based biodiesel provide a good alternative however, high cost associated with maintenance such as fertilizers, availability of farmland and fresh water restrict their uses on pilot scale. Microbes derived single cell oil (SCO) being seen as a promising substitute in coming years. While, requirement of fresh water for their proper cell metabolism is major hurdle. In line, current study addresses the aforementioned problems and established a newly isolate of R. glutinis ISO A1 in sea water and wastewater medium for enhanced lipid accumulation. The strategy not only curtail the allied media cost but also provide an eco-friendly approach that can be applied at industrial level. Oleaginous yeast, R. glutinis accumulated a high quantity of total lipid up to 3.14 ± 0.15 g/L with 23.55 ± 0.76 % of lipid content on cell dry weight basis in its cellular compartment. Long chain monounsaturated (73.41 percent) was found in the FAME profile, which helps enhance the quality of the biodiesel at low temperatures (such as CFPP), as well as its oxidative stability and cetane number, in accordance with ASTMD6751-02 and EN-14214 requirements.