NANO MATERIAL BASED SENSORS FOR THE DETERMINATION OF ASPIRIN METABOLITES

Abstract

A graphene modified glassy carbon (GRIGCE) sensor has been developed for the determination of aspirin metabolites, 2,3- and 2,5-dihydroxybenzoic acids (2,3- and 2,5-DHB). The modified sensor was characterized by Field Emission Scanning Ilectron Microscopy and Electrochemical impedance Spectroscopy. The electrochemical behavior of 2,3- and 2,5-DHB was investigated by cyclic and square wave voltammetry. The modified sensor exhibited excellent electrocatalytic activity for the oxidation of 2,3- and 2,5-DHB, leading to a remarkable enhancement in the peak current as compared to the bare sensor. The results were attributed to the enhanced surface area and high conductivity of GR. The anodic peak currents of 2,3- and 2,5-DHB were found to be linear in the concentration range of 1-150 jiM and 1-200 jiM with the detection limit of 47 nM and 51 nM, respectively. The sensor was capable to determine 2,5-Di-IB effectively without any interference from the uric acid and other metabolites present in the urine samples. The practical utility of GRIGCE has been successliilly demonstrated for the determination of 2,5-DuB in the urine samples of persons undergoing treatment with aspirin.