CONTROLLED RELEASE OF AMPHOTERICIN B FROM ELECTROSPUN NANOFIBER BASED ON GELATIN: A NEW INSIGHT TO DRUG DELIVERY AGAINST LESHMANIASIS

Abstract

Electrospun nanofibers were developed as a new system for the delivery of a medical drug amphotericin B. In this study drug loaded nanofibers of average diameter of 100nm were fabricated from a natural biodegradable polymer based on gelatin by advanced electrospinning process. The fibers in our research were prepared from 10 wt. % gelatin in acetic acid and water mixed with 1. wt. % amphotericin B and characterized by the Field Emission Scanning Electron Microscopy (FE-SEM) for observation of fiber morphology. The chemical integrity of the drug in electrospun nanofibers were confirmed by Fourier Transformation Infra Red (FTIR) spectroscopic analysis and the release of the amphotericin B from the electrospun fibers were further investigated by UV-Vis spectrophotometer in phosphate buffer of PH 7.4 at normal body temperature of 37°C. The release profile suggests that electrospun amphotericin B loaded biodegradable micro and nanofibers may be promising for the treatment of leshmaniasis and several sever fungal infections as alternative drug delivery devices.