SYNTHESIS OF METAL OXIDE NANOPARTICLES AND THEIR INHIBITORY ACTION FOR GLYCOSIDE HYDROLASES

Abstract

The present report describes the synthesis of water soluble biocompatible metal oxides nanoparticles in aqueous media. These particles have been characterized by using various spectroscopic and electron microscopy. Advanced tools like UV- Visible, emission, FT-IR, AFM and FESEM have been employed for their characterization. These particles depict the onset absorption about 320 nm and exhibit band gap emission at 377 nm has been employed for their characterization. These features are fairly different to its bulk material and exhibit size quantization effect. By using Scherrer formula the particles size for the two studied nanosystems is observed to lie between to 10 — 12 nm. As synthesized nanosystems were examined to control the glycosidases activity in reference to develop a substitute for the standard drug having side effects. The inhibitory action of SP 1 and SP2 nanoparticles with glycosidases was observed to be 61% and 72% respectively. Moreover, the inhibition activity of the studied nanosystems (SP 1) was found to be very similar to that of standard drug being used for diabetes. The coating of these particles with drug demonstrated an enhancement in inhibition activity of the enzyme by about 30 %. To the best of our knowledge the first work describing the synthesis of drug capped nanoparticles and demonstrating the inhibition activity of enzyme both nanoparticles as well as drug coated nanoparticles.